Dhx9 atpase assay

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August undefined, 2024

WebJun 4, 2013 · Various results, including in vitro assays, show that the FG/GLFG region of Nup98 binds to N- and C-terminal regions of DHX9 in an RNA facilitated manner. Importantly, binding of Nup98 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated transcription. WebMay 8, 2024 · Because DHX9-depleted cells exhibit a termination defect (Figures 4 H and 4I), we examined the potential role of DHX9 in preventing R-loop-associated DNA damage. For these studies, we used the Top1 inhibitor camptothecin (CPT), which is known to promote R-loop accumulation and replication- and transcription-associated DNA damage …

The ATPase/helicase activity of DHX9 is required for

WebNov 1, 2011 · DHX9 is a member of the DExDc helicase family and is a homolog of Drosophila maleless protein, which is essential to regulate X-linked gene expression and the survival of male larvae ().DHX9 … WebNov 2, 2024 · Much interest has been expended lately in characterizing the association between DExH-Box helicase 9 (DHX9) dysregulation and malignant development, however, the enigmatic nature of DHX9 has … dark fate cast

Establishment of a Primary Screening Assay for the DHX9 Helicase ...

WebDHX9. On the top, protein expression in current human tissue, based on all annotated cell types, is reported with the units not detected (n), low (l), medium (m) and high (h). … WebMar 29, 2024 · d, Luciferase assays show that DHX9 depletion significantly reduces expression of inverted-repeat-Alu-containing 3′ UTR elements, which can be alleviated with wild-type (WT) DHX9 overexpression ... WebImportantly, binding of Nup98 to DHX9 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated … dark father star wars

DHX9 Pairs with IPS-1 To Sense Double-Stranded RNA …

DHX9 Gene - GeneCards DHX9 Protein DHX9 Antibody

WebJul 23, 2010 · The SAT motif has been shown by mutagenesis to uncouple ATPase activity from RNA helicase activity in both DEAD-box 46, 47, ... NTPase assay. DHX9 325–840 was diluted into an assay buffer [20 mM Hepes (pH 7.5), 3 mM MgCl 2, 300 mM NaCl, 10% glycerol, and 2 mM TCEP] up to final concentrations of 7–80 nM. The assay was … WebCCK-8 assay showed that DHX9 knockdown significantly inhibited the proliferation of MHCC97H cells at 48, 72, and 96 h (Figure 4B). In the scratch assay, DHX9 siRNA-treated cells covered almost 73% of scratches in 24 h and at least 85% in 48 h, while the cells in the NC group covered only 18% in 24 h and 25% in 48 h (Figure 4C,D). bishop afc twitterWebJul 5, 2024 · We show that DHX9 plays a critical role in HR by promoting the recruitment of BRCA1 to RNA as part of the RNA Polymerase II transcription complex, where it … bishop after hours

"WebNov 13, 2024 · The proliferation rates of DHX9-overexpressed or -silenced CRC cells were determined by MTS assay and trypan blue exclusion assay. We found that there was no … " - Dhx9 atpase assay

Dhx9 atpase assay

Human DHX9 helicase preferentially unwinds RNA ... - ScienceDirect

WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ... WebProduct overview. ATPase Assay Kit (Colorimetric) (ab234055) provides a quick and easy method for monitoring ATPase activity in cell and tissue lysates. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, in which phosphate reacts with malachite green, a strong, stable chromophore is ...

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WebAug 1, 2024 · The complex biology of DHX9 was recently reviewed [221]. Helicase and competition assays proved DHX9 to bind and resolve G4s with stronger kinetics toward rG4 and strict ATP-dependence in vitro, as confirmed by the complete loss of G4-unwinding function upon ATPase inhibition by N-ethylmaleimide poisoning [222]. WebJ Immunol. Author manuscript; available in PMC 2013 May 17. Published in final edited form as: J Immunol. 2011 Nov 1; 187(9): 4501–4508.

WebJul 23, 2010 · Competition assays were consistent with these findings as all four NTPs inhibited DHX9 ATPase activity (Fig. 1D) [33]. Taken together, these results indicate that our recombinant DHX9 preparation ... WebSep 17, 2013 · Our ChIP assays indicate that DHX9 was stably bound to the H-DNA-forming region of the c-MYC promoter sequence. Thus, it is likely that the ChIP assays captured specific interactions between DHX9 and H-DNA in chromatin-plasmid complexes in human cells. ... FANCJ uses its motor ATPase to destabilize protein-DNA complexes, …

WebTwo NDD-associated variants, p.(Gly411Glu) and p.(Arg761Gln), altered DHX9 ATPase activity. The severe NDD-associated variant p.(Arg141Gln) did not impact DHX9 localization but instead increased R ... WebOct 19, 2015 · The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from …

WebJun 10, 2011 · For competition assays, unlabeled competitor substrates (D-loops, R-loops, forks and G4 structures) were preincubated with DHX9 for 3 min at 25 °C in helicase …

WebJun 6, 2024 · The RNA helicase DHX9 associates with IGS-rRNA pRNA is a 250–300 nucleotide transcript that derives from processing of the 2-kb … darkfathom cave oblivionWebMar 21, 2024 · Entrez Gene Summary for DHX9 Gene. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that … bishop african violetWebKeywords: ATPase assay, ATA, aurintricarboxylic acid, DHX9, HTS, RNA binding, RNA helicase. Abstract: The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional ... bishop against bishopWebWe show that aurintricarboxylic acid, a promiscuous protein-nucleic acid inhibitor prevents DHX9-mediated hydrolysis demonstrating that the assay is also capable of detecting … bishop agnes bishop against popeWebMar 6, 2024 · ATPase Assays. One of the most robust uses of measuring ATPase inhibition is with an ATPase Activity Assay. The protocol developed by BellBrook Labs has been in use for over ten years, with … dark faucet with stainless sinkWebDHX9 is part of cluster 34 Non-specific - Transcription with confidence i Confidence is the fraction of times a gene was assigned to the cluster in repeated clustering, and therefore … bishop agnelo gracias