Ribofecttm
Webb15 mars 2024 · The cells were then transfected with shRNAs using riboFECTTM CP reagent (RiboBio, China) at final shRNA concentrations of 50 nM in accordance with the manufacturer’s recommended protocols. The shRNA sequences used to knock-down PECAM1 were: sense 5′-GAA UUC UCG AGA CCA GAA UUU and antisense 5′-AUU CUG … WebbCN109536586B CN202411585690.6A CN202411585690A CN109536586B CN 109536586 B CN109536586 B CN 109536586B CN 202411585690 A CN202411585690 A CN 202411585690A CN 109536586 B CN109536586 B CN 109536586B Authority CN China Prior art keywords rat secretion mirna adenohypophysis mir Prior art date 2024-12-25 …
Ribofecttm
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Webb25 sep. 2024 · LncRNAL20992-siRNA was diluted with RiboFECT CP buffer, and transfection reagent (TR) (RiboFECTTM CP transfection kit; RIBOBIO, Guangzhou, China) was added to the plates and mixed evenly, followed by incubation at room temperature for 10 min. Cells which transfected in this research were incubated for 48 h after … WebbLipofectamine™ MessengerMAX™ reagent is designed to transfect a higher amount of mRNA into neurons and a broad spectrum of difficult-to-transfect primary cells. This …
WebbResearch Article miR-876 Inhibits EMT and Liver Fibrosis via POSTN to Suppress Metastasis in Hepatocellular Carcinoma Kai Chen,1,2 Zhonghu Li,3 Mengyun Zhang,4 Bo Wang,3 Tao Peng,5 Yanbing Shen,3 Jianxin Zhang,3 Jiaxin Ye,3 Yu Liu,2 Di Tang,3 Minjie Peng,6 Dandan Ma,3 Zhengkang Xiao,3 Yujun Zhang,1 Weidong Jin,3 and Xiaowu Li 1,6 … Webb16 feb. 2024 · To silence the expression of CCL2, we inoculated PC-12 cells in 6-well plates containing appropriate amounts of complete medium. When the cell density reached 30–50%, transfection was performed as per the instructions of the riboFECTTM CP transfection kit (RiboBio, China) with a working concentration of 50 nM of Si-RNA.
Webb20 okt. 2024 · 1 INTRODUCTION. D-Glucose is an important fuel for the metabolic engines in the body and provides the main energy source for the critical activities of human life (Kowalski & Bruce, 2014; Zierler, 1999).D-Glucose is first absorbed through the small intestinal epithelial cells (IEC) and then transported to the bloodstream and its … WebbCell proliferation analysis. After transfection for 24h, ES cells were seeded in a 96-well plate at a density of 1000 cells per well. Then, cells in each well were incubated with 10ml of …
Webb10 feb. 2024 · Human MSCs were incubated with antibiotics-free CCM in twenty 150 cm 2 cell culture plates and transfected directly with miR-146a-5p inhibitor or miRNA scramble control using riboFECTTM CP Transfection Kit for 48 h after the cell density reached about 50% confluency.
WebbRibofecttm Cp Transfection Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … fab biscuits history facts point 8WebbsiRNA (siCtrl) and riboFECTTM CP Transfection kit were purchased from RiboBio Co., Ltd. (Guangzhou, China). Cellular ROS Detection Reagent (CM-H2DCFDA) was purchased from Invitrogen (Thermo Fisher Scientific Inc., Waltham, MA, US). Mouse IL-1β ELISA Kit was purchased from MultiSciences(Lianke)Biotech Co., Ltd. (Hangzhou, China). does holding an action use your reactionWebbCells were seeded into 6-well plates at 37 °C, and after 24 h of culture, cells were transfected with PDGFRβ siRNA (5 μL, 20 μmol/L) or control using riboFECTTM CP … fabbitheapple